PrimaStem

Impression

The Institute for Research on Stem Cells and Brain in Lyon (SBRI) studies the development of neuronal circuits in normal and pathological conditions, in particular in Parkinson’s disease.

PrimaStem is a biotechnology platform on stem cells  funded by INSERM and INRA in the UMRS 846. The goal of PrimaStem within INGESTEM is to generate ES/ IPSC-based germline chimaeras in non-human primates, rabbits, and rodents for transgenic models.

Management:

  • Scientific Direction: Dr. Pierre Savatier
  • Technical manager: Dr. Marielle Afanassieff

Expertise:

  • Engineering non murine pluripotent stem cells.
  • Molecular mechanisms involved in self-renewal in the naive pluripotent state.
  • Animal transgenesis.

Involvement in INGESTEM:

  • Production of somatic and germline chimeras in different species including rabbits and non-human primates from ES and iPS cell lines.
  • Generation of pluripotent stem cell lines that self-renew in naive pluripotent state (study of early development)
  • Construction of IPSC with gene reporter for monitoring the behavior of grafted cells in vivo (safety and efficacy of cell therapies).

Major publications:

  • Reinforcement of STAT3 activity reprogrammed human embryonic stem cells to pluripotency-like naive. Chen H, Aksoy I Gonnot F, Osteil P Aubry M, Hamela C Rognard C. Hochard A Neighbor S, Fontaine E, Mure million Afanassieff million Cleroux E, Guibert S, Chen J, Vallot C Acloque H , C. Genthon, Donnadieu C. De Vos J, Sanlaville D Guerin JF, Weber M, Stanton LW, C Rougeulle Bread B, PY Bourillot, Savatier P. Nat Commun. 2015 May 13; 6: 7095.
  • Generation of Embryonic Stem Cells in Rabbits. Afanassieff M, P Osteil, Savatier P. Methods Mol Biol. 12 March 2015.
    Klf4 and KLF5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells. Aksoy I Giudice V, Delahaye E, Wianny F Aubry M, Mure M, Chen J, Jauch R, Bogu GK, Nolden T Himmelbauer H, Xavier Doss M, Sachinidis A, Schulz H, Hummel O, Martinelli P, Hübner N, Stanton LW, Real FX, Bourillot PY, Savatier P. Nat Commun. 2014 Apr 28; 5: 3719.
  • Induced pluripotent stem cells derived from rabbits Some exhibit characteristics of pluripotency naive. Osteil P Tapponnier Y, Markossian S, Bucket M Schmaltz-Panel B, Jouneau L, Cabau C. Joly T, Blachere T Gócza E, Bernat A, Yerle million Acloque H, Hidot S, Bosze Z Duranthon V Savatier P, M. Biol Afanassieff Open. 10 May 2013; 2 (6): 613-28.
  • Transgenic rhesus monkeys produced by gene transfer into early cleavage embryos using internship has simian immunodeficiency virus-based vector. Niu Y., Y. Yu, A. Bernat, S. Yang, X. He, X. Guo, D. Chen, Y. Chen, Ji S., W. If, T. Tan, Q. Wei, Wang H ., L. Shi, Afanassieff M., P. * Savatier, Q. Zhou *, and * Ji W. (2010) Proc Natl Acad Sci USA. 107: 17663-7 [* co-senior investigators].
  • A short G1 is an intrinsic determinant of artless embryonic stem cell pluripotency. Coronado D., M. Godet, Bourillot P.-Y., Tapponnier Y., A. Bernat, Mr. Petit, Mr. Afanassieff, Markossian S., A. Malashicheva, Iacone R., K. Anastassiadis, and Savatier P . (2013). Stem Cell Res. 10: 118-31.
  • Molecular and functional features demarcate embryonic and induced pluripotent stem cells in rabbits. Osteil P., Y. Tapponnier, Markossian S., M. Godet, Schmaltz-Panel B., L. Jouneau, Cabau, C. Joly, Blachere T., Gozca, E., A. Bernat, Yerle M., Acloque H., S. Hidot, Bözse, Z., V. Duranthon, Savatier P. *, and * Afanassieff M. (2013). Open Biology. 2: 613-28. * Co-senior authors.

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